Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
A reliable method for the separation of free and esterified cholesterol in adipocyte extracts is described. The procedure uses Sephadex LH-20, a lipophilic dextran gel, with a solvent system of chloroform-hexane 55:45 (v/v). Interference by excess triglyceride, such as that encountered in adipocyte...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1978-08-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520412805 |