Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.

Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.

A reliable method for the separation of free and esterified cholesterol in adipocyte extracts is described. The procedure uses Sephadex LH-20, a lipophilic dextran gel, with a solvent system of chloroform-hexane 55:45 (v/v). Interference by excess triglyceride, such as that encountered in adipocyte...

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Bibliographic Details
Main Authors: B R Krause, A D Hartman
Format: Article
Language:English
Published: Elsevier 1978-08-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520412805
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spelling doaj-4cd54cbcced14cfdbe7d4616888b55142021-04-24T05:53:26ZengElsevierJournal of Lipid Research0022-22751978-08-01196774777Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.B R KrauseA D HartmanA reliable method for the separation of free and esterified cholesterol in adipocyte extracts is described. The procedure uses Sephadex LH-20, a lipophilic dextran gel, with a solvent system of chloroform-hexane 55:45 (v/v). Interference by excess triglyceride, such as that encountered in adipocyte total lipid extracts, was not observed, and overall recovery of both sterols exceeded 98%.http://www.sciencedirect.com/science/article/pii/S0022227520412805
collection DOAJ
language English
format Article
sources DOAJ
author B R Krause
A D Hartman
spellingShingle B R Krause
A D Hartman
Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
Journal of Lipid Research
author_facet B R Krause
A D Hartman
author_sort B R Krause
title Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
title_short Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
title_full Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
title_fullStr Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
title_full_unstemmed Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
title_sort quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1978-08-01
description A reliable method for the separation of free and esterified cholesterol in adipocyte extracts is described. The procedure uses Sephadex LH-20, a lipophilic dextran gel, with a solvent system of chloroform-hexane 55:45 (v/v). Interference by excess triglyceride, such as that encountered in adipocyte total lipid extracts, was not observed, and overall recovery of both sterols exceeded 98%.
url http://www.sciencedirect.com/science/article/pii/S0022227520412805
work_keys_str_mv AT brkrause quantificationofadipocytefreeandesterifiedcholesterolusingliquidgelchromatography
AT adhartman quantificationofadipocytefreeandesterifiedcholesterolusingliquidgelchromatography
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