<i>Cucumis melo</i> L. Germplasm in Tunisia: Unexploited Sources of Resistance to Fusarium Wilt
Breeding for disease resistance has been one of the most important research objectives in melon for the last few decades. Fusarium wilt caused by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> (<i>Fom</i>) is among the most threatening melon fungal diseases along...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-07-01
|
Series: | Horticulturae |
Subjects: | |
Online Access: | https://www.mdpi.com/2311-7524/7/8/208 |
id |
doaj-4cfb0694f74f4f26809fc7209943a787 |
---|---|
record_format |
Article |
spelling |
doaj-4cfb0694f74f4f26809fc7209943a7872021-08-26T13:48:08ZengMDPI AGHorticulturae2311-75242021-07-01720820810.3390/horticulturae7080208<i>Cucumis melo</i> L. Germplasm in Tunisia: Unexploited Sources of Resistance to Fusarium WiltHela Chikh-Rouhou0Maria Luisa Gómez-Guillamón1Vicente González2Rafika Sta-Baba3Ana Garcés-Claver4Regional Research Centre on Horticulture and Organic Agriculture (CRRHAB), LR21AGR03, University of Sousse, Sousse 4042, TunisiaInstituto de Hortofruticultura Subtropical y Mediterránea-La Mayora, UMA-CSIC, 29750 Algarrobo, SpainCentro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón—IA2 (CITA-Universidad de Zaragoza), Avda. Montañana 930, 50059 Zaragoza, SpainRegional Research Centre on Horticulture and Organic Agriculture (CRRHAB), LR21AGR03, University of Sousse, Sousse 4042, TunisiaCentro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón—IA2 (CITA-Universidad de Zaragoza), Avda. Montañana 930, 50059 Zaragoza, SpainBreeding for disease resistance has been one of the most important research objectives in melon for the last few decades. Fusarium wilt caused by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> (<i>Fom</i>) is among the most threatening melon fungal diseases along the Mediterranean coast, affecting yield and quality. Since genetic resistance is one of the best sustainable strategies that can be used to control this pathogen, 27 Tunisian melon accessions collected from local farmers have been tested using phenotypic and molecular approaches to identify new sources of resistance to be used directly as cultivars in affected areas or as resistance donors in breeding programs. The phenotypic evaluations, using artificial inoculations, showed several resistant accessions to the pathogenic races of <i>Fom</i>. Additionally, molecular analysis revealed that 13 out of 27 accessions carried the resistance <i>Fom</i>-1 gene (7 in homozygous state and 6 in heterozygous state), confirming their resistance to races 0 and 2. Two of them were also identified as heterozygous for the <i>Fom</i>-2 gene, being resistant to races 0 and 1. Furthermore, two accessions with a high level of resistance to the most virulent race 1.2 have been also reported. This melon germplasm should be explored as a potential source of resistance genes in breeding programs to develop new resistant melon cultivars.https://www.mdpi.com/2311-7524/7/8/208melonbreeding programgenetic resistance<i>Fom-</i>1 and <i>Fom-</i>2 genesmolecular markers<i>Fom</i> race 1.2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hela Chikh-Rouhou Maria Luisa Gómez-Guillamón Vicente González Rafika Sta-Baba Ana Garcés-Claver |
spellingShingle |
Hela Chikh-Rouhou Maria Luisa Gómez-Guillamón Vicente González Rafika Sta-Baba Ana Garcés-Claver <i>Cucumis melo</i> L. Germplasm in Tunisia: Unexploited Sources of Resistance to Fusarium Wilt Horticulturae melon breeding program genetic resistance <i>Fom-</i>1 and <i>Fom-</i>2 genes molecular markers <i>Fom</i> race 1.2 |
author_facet |
Hela Chikh-Rouhou Maria Luisa Gómez-Guillamón Vicente González Rafika Sta-Baba Ana Garcés-Claver |
author_sort |
Hela Chikh-Rouhou |
title |
<i>Cucumis melo</i> L. Germplasm in Tunisia: Unexploited Sources of Resistance to Fusarium Wilt |
title_short |
<i>Cucumis melo</i> L. Germplasm in Tunisia: Unexploited Sources of Resistance to Fusarium Wilt |
title_full |
<i>Cucumis melo</i> L. Germplasm in Tunisia: Unexploited Sources of Resistance to Fusarium Wilt |
title_fullStr |
<i>Cucumis melo</i> L. Germplasm in Tunisia: Unexploited Sources of Resistance to Fusarium Wilt |
title_full_unstemmed |
<i>Cucumis melo</i> L. Germplasm in Tunisia: Unexploited Sources of Resistance to Fusarium Wilt |
title_sort |
<i>cucumis melo</i> l. germplasm in tunisia: unexploited sources of resistance to fusarium wilt |
publisher |
MDPI AG |
series |
Horticulturae |
issn |
2311-7524 |
publishDate |
2021-07-01 |
description |
Breeding for disease resistance has been one of the most important research objectives in melon for the last few decades. Fusarium wilt caused by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> (<i>Fom</i>) is among the most threatening melon fungal diseases along the Mediterranean coast, affecting yield and quality. Since genetic resistance is one of the best sustainable strategies that can be used to control this pathogen, 27 Tunisian melon accessions collected from local farmers have been tested using phenotypic and molecular approaches to identify new sources of resistance to be used directly as cultivars in affected areas or as resistance donors in breeding programs. The phenotypic evaluations, using artificial inoculations, showed several resistant accessions to the pathogenic races of <i>Fom</i>. Additionally, molecular analysis revealed that 13 out of 27 accessions carried the resistance <i>Fom</i>-1 gene (7 in homozygous state and 6 in heterozygous state), confirming their resistance to races 0 and 2. Two of them were also identified as heterozygous for the <i>Fom</i>-2 gene, being resistant to races 0 and 1. Furthermore, two accessions with a high level of resistance to the most virulent race 1.2 have been also reported. This melon germplasm should be explored as a potential source of resistance genes in breeding programs to develop new resistant melon cultivars. |
topic |
melon breeding program genetic resistance <i>Fom-</i>1 and <i>Fom-</i>2 genes molecular markers <i>Fom</i> race 1.2 |
url |
https://www.mdpi.com/2311-7524/7/8/208 |
work_keys_str_mv |
AT helachikhrouhou icucumismeloilgermplasmintunisiaunexploitedsourcesofresistancetofusariumwilt AT marialuisagomezguillamon icucumismeloilgermplasmintunisiaunexploitedsourcesofresistancetofusariumwilt AT vicentegonzalez icucumismeloilgermplasmintunisiaunexploitedsourcesofresistancetofusariumwilt AT rafikastababa icucumismeloilgermplasmintunisiaunexploitedsourcesofresistancetofusariumwilt AT anagarcesclaver icucumismeloilgermplasmintunisiaunexploitedsourcesofresistancetofusariumwilt |
_version_ |
1721193026651422720 |