A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation
Adaptation of glioblastoma to caloric restriction induces compensatory changes in tumor metabolism that are incompletely known. Here we show that in human glioblastoma cells maintained in exhausted medium, SHC adaptor protein 3 (SHC3) increases due to down-regulation of SHC3 protein degradation. Thi...
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doaj-4cfc31f7fb3c4015820e036756e306a92020-11-25T02:26:34ZengMDPI AGCells2073-44092020-05-0191249124910.3390/cells9051249A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose StarvationAlberto Azzalin0Francesca Brambilla1Eloisa Arbustini2Katia Basello3Attilio Speciani4Pierluigi Mauri5Paola Bezzi6Lorenzo Magrassi7Neurosurgery, Dipartimento di Scienze Clinico-Chirurgiche e Pediatriche, Università degli Studi di Pavia, Fondazione IRCCS Policlinico S. Matteo, 27100 Pavia, ItalyProteomics and Metabolomics Institute for Biomedical Technologies (ITB-CNR), Segrate, 20090 Milan, ItalyMolecular Genetic Laboratory-Transplant Research Area, Fondazione IRCCS Policlinico S. Matteo, 27100 Pavia, ItalyCryolab, University of Rome Tor Vergata, 00133 Rome, ItalyCryolab, University of Rome Tor Vergata, 00133 Rome, ItalyProteomics and Metabolomics Institute for Biomedical Technologies (ITB-CNR), Segrate, 20090 Milan, ItalyDépartement des Neurosciences Fondamentales, Université de Lausanne, 1005 Lausanne, SwitzerlandNeurosurgery, Dipartimento di Scienze Clinico-Chirurgiche e Pediatriche, Università degli Studi di Pavia, Fondazione IRCCS Policlinico S. Matteo, 27100 Pavia, ItalyAdaptation of glioblastoma to caloric restriction induces compensatory changes in tumor metabolism that are incompletely known. Here we show that in human glioblastoma cells maintained in exhausted medium, SHC adaptor protein 3 (SHC3) increases due to down-regulation of SHC3 protein degradation. This effect is reversed by glucose addition and is not present in normal astrocytes. Increased SHC3 levels are associated to increased glucose uptake mediated by changes in membrane trafficking of glucose transporters of the solute carrier 2A superfamily (GLUT/SLC2A). We found that the effects on vesicle trafficking are mediated by SHC3 interactions with adaptor protein complex 1 and 2 (AP), BMP-2-inducible protein kinase and a fraction of poly ADP-ribose polymerase 1 (PARP1) associated to vesicles containing GLUT/SLC2As. In glioblastoma cells, PARP1 inhibitor veliparib mimics glucose starvation in enhancing glucose uptake. Furthermore, cytosol extracted from glioblastoma cells inhibits PARP1 enzymatic activity in vitro while immunodepletion of SHC3 from the cytosol significantly relieves this inhibition. The identification of a new pathway controlling glucose uptake in high grade gliomas represents an opportunity for repositioning existing drugs and designing new ones.https://www.mdpi.com/2073-4409/9/5/1249glioblastoma cellsaerobic glycolysisGLUT/SLC2ASHC3PARP1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alberto Azzalin Francesca Brambilla Eloisa Arbustini Katia Basello Attilio Speciani Pierluigi Mauri Paola Bezzi Lorenzo Magrassi |
spellingShingle |
Alberto Azzalin Francesca Brambilla Eloisa Arbustini Katia Basello Attilio Speciani Pierluigi Mauri Paola Bezzi Lorenzo Magrassi A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation Cells glioblastoma cells aerobic glycolysis GLUT/SLC2A SHC3 PARP1 |
author_facet |
Alberto Azzalin Francesca Brambilla Eloisa Arbustini Katia Basello Attilio Speciani Pierluigi Mauri Paola Bezzi Lorenzo Magrassi |
author_sort |
Alberto Azzalin |
title |
A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation |
title_short |
A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation |
title_full |
A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation |
title_fullStr |
A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation |
title_full_unstemmed |
A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation |
title_sort |
new pathway promotes adaptation of human glioblastoma cells to glucose starvation |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2020-05-01 |
description |
Adaptation of glioblastoma to caloric restriction induces compensatory changes in tumor metabolism that are incompletely known. Here we show that in human glioblastoma cells maintained in exhausted medium, SHC adaptor protein 3 (SHC3) increases due to down-regulation of SHC3 protein degradation. This effect is reversed by glucose addition and is not present in normal astrocytes. Increased SHC3 levels are associated to increased glucose uptake mediated by changes in membrane trafficking of glucose transporters of the solute carrier 2A superfamily (GLUT/SLC2A). We found that the effects on vesicle trafficking are mediated by SHC3 interactions with adaptor protein complex 1 and 2 (AP), BMP-2-inducible protein kinase and a fraction of poly ADP-ribose polymerase 1 (PARP1) associated to vesicles containing GLUT/SLC2As. In glioblastoma cells, PARP1 inhibitor veliparib mimics glucose starvation in enhancing glucose uptake. Furthermore, cytosol extracted from glioblastoma cells inhibits PARP1 enzymatic activity in vitro while immunodepletion of SHC3 from the cytosol significantly relieves this inhibition. The identification of a new pathway controlling glucose uptake in high grade gliomas represents an opportunity for repositioning existing drugs and designing new ones. |
topic |
glioblastoma cells aerobic glycolysis GLUT/SLC2A SHC3 PARP1 |
url |
https://www.mdpi.com/2073-4409/9/5/1249 |
work_keys_str_mv |
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