A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus.
Plus-stranded RNA viruses replicate in infected cells by assembling viral replicase complexes consisting of viral- and host-coded proteins. Previous genome-wide screens with Tomato bushy stunt tombusvirus (TBSV) in a yeast model host revealed the involvement of seven ESCRT (endosomal sorting complex...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2009-12-01
|
Series: | PLoS Pathogens |
Online Access: | http://europepmc.org/articles/PMC2791863?pdf=render |
id |
doaj-4d18b37f63134e349813223902deb47b |
---|---|
record_format |
Article |
spelling |
doaj-4d18b37f63134e349813223902deb47b2020-11-24T22:09:19ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742009-12-01512e100070510.1371/journal.ppat.1000705A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus.Daniel BarajasYi JiangPeter D NagyPlus-stranded RNA viruses replicate in infected cells by assembling viral replicase complexes consisting of viral- and host-coded proteins. Previous genome-wide screens with Tomato bushy stunt tombusvirus (TBSV) in a yeast model host revealed the involvement of seven ESCRT (endosomal sorting complexes required for transport) proteins in viral replication. In this paper, we show that the expression of dominant negative Vps23p, Vps24p, Snf7p, and Vps4p ESCRT factors inhibited virus replication in the plant host, suggesting that tombusviruses co-opt selected ESCRT proteins for the assembly of the viral replicase complex. We also show that TBSV p33 replication protein interacts with Vps23p ESCRT-I and Bro1p accessory ESCRT factors. The interaction with p33 leads to the recruitment of Vps23p to the peroxisomes, the sites of TBSV replication. The viral replicase showed reduced activity and the minus-stranded viral RNA in the replicase became more accessible to ribonuclease when derived from vps23Delta or vps24Delta yeast, suggesting that the protection of the viral RNA is compromised within the replicase complex assembled in the absence of ESCRT proteins. The recruitment of ESCRT proteins is needed for the precise assembly of the replicase complex, which might help the virus evade recognition by the host defense surveillance system and/or prevent viral RNA destruction by the gene silencing machinery.http://europepmc.org/articles/PMC2791863?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniel Barajas Yi Jiang Peter D Nagy |
spellingShingle |
Daniel Barajas Yi Jiang Peter D Nagy A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus. PLoS Pathogens |
author_facet |
Daniel Barajas Yi Jiang Peter D Nagy |
author_sort |
Daniel Barajas |
title |
A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus. |
title_short |
A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus. |
title_full |
A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus. |
title_fullStr |
A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus. |
title_full_unstemmed |
A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus. |
title_sort |
unique role for the host escrt proteins in replication of tomato bushy stunt virus. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2009-12-01 |
description |
Plus-stranded RNA viruses replicate in infected cells by assembling viral replicase complexes consisting of viral- and host-coded proteins. Previous genome-wide screens with Tomato bushy stunt tombusvirus (TBSV) in a yeast model host revealed the involvement of seven ESCRT (endosomal sorting complexes required for transport) proteins in viral replication. In this paper, we show that the expression of dominant negative Vps23p, Vps24p, Snf7p, and Vps4p ESCRT factors inhibited virus replication in the plant host, suggesting that tombusviruses co-opt selected ESCRT proteins for the assembly of the viral replicase complex. We also show that TBSV p33 replication protein interacts with Vps23p ESCRT-I and Bro1p accessory ESCRT factors. The interaction with p33 leads to the recruitment of Vps23p to the peroxisomes, the sites of TBSV replication. The viral replicase showed reduced activity and the minus-stranded viral RNA in the replicase became more accessible to ribonuclease when derived from vps23Delta or vps24Delta yeast, suggesting that the protection of the viral RNA is compromised within the replicase complex assembled in the absence of ESCRT proteins. The recruitment of ESCRT proteins is needed for the precise assembly of the replicase complex, which might help the virus evade recognition by the host defense surveillance system and/or prevent viral RNA destruction by the gene silencing machinery. |
url |
http://europepmc.org/articles/PMC2791863?pdf=render |
work_keys_str_mv |
AT danielbarajas auniqueroleforthehostescrtproteinsinreplicationoftomatobushystuntvirus AT yijiang auniqueroleforthehostescrtproteinsinreplicationoftomatobushystuntvirus AT peterdnagy auniqueroleforthehostescrtproteinsinreplicationoftomatobushystuntvirus AT danielbarajas uniqueroleforthehostescrtproteinsinreplicationoftomatobushystuntvirus AT yijiang uniqueroleforthehostescrtproteinsinreplicationoftomatobushystuntvirus AT peterdnagy uniqueroleforthehostescrtproteinsinreplicationoftomatobushystuntvirus |
_version_ |
1725812508619440128 |