An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and...
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doaj-4d227e8c4551426a9bfdab2c027144152021-02-02T08:42:49ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2018-09-0133186195An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway designTing Shi0Pingping Han1Chun You2Yi-Heng P. Job Zhang3Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaCorresponding author.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaCorresponding author..; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaAlthough most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes, has been proposed for low-cost biomanufacturing of bioenergy, food, biochemicals, and nutraceuticals. In addition to the most important advantage-high product yield, in vitro synthetic biology platform features several other biomanufacturing advantages, such as fast reaction rate, easy product separation, open process control, broad reaction condition, tolerance to toxic substrates or products, and so on. In this article, we present the basic bottom-up design principles of in vitro synthetic pathway from basic building blocks-BioBricks (thermoenzymes and/or immobilized enzymes) to building modules (e.g., enzyme complexes or multiple enzymes as a module) with specific functions. With development in thermostable building blocks-BioBricks and modules, the in vitro synthetic biology platform would open a new biomanufacturing age for the cost-competitive production of biocommodities. Keywords: Biomanufacturing, Coenzyme regeneration, Pathway design, In vitro synthetic biologyhttp://www.sciencedirect.com/science/article/pii/S2405805X18300255 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ting Shi Pingping Han Chun You Yi-Heng P. Job Zhang |
spellingShingle |
Ting Shi Pingping Han Chun You Yi-Heng P. Job Zhang An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design Synthetic and Systems Biotechnology |
author_facet |
Ting Shi Pingping Han Chun You Yi-Heng P. Job Zhang |
author_sort |
Ting Shi |
title |
An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design |
title_short |
An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design |
title_full |
An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design |
title_fullStr |
An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design |
title_full_unstemmed |
An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design |
title_sort |
in vitro synthetic biology platform for emerging industrial biomanufacturing: bottom-up pathway design |
publisher |
KeAi Communications Co., Ltd. |
series |
Synthetic and Systems Biotechnology |
issn |
2405-805X |
publishDate |
2018-09-01 |
description |
Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes, has been proposed for low-cost biomanufacturing of bioenergy, food, biochemicals, and nutraceuticals. In addition to the most important advantage-high product yield, in vitro synthetic biology platform features several other biomanufacturing advantages, such as fast reaction rate, easy product separation, open process control, broad reaction condition, tolerance to toxic substrates or products, and so on. In this article, we present the basic bottom-up design principles of in vitro synthetic pathway from basic building blocks-BioBricks (thermoenzymes and/or immobilized enzymes) to building modules (e.g., enzyme complexes or multiple enzymes as a module) with specific functions. With development in thermostable building blocks-BioBricks and modules, the in vitro synthetic biology platform would open a new biomanufacturing age for the cost-competitive production of biocommodities. Keywords: Biomanufacturing, Coenzyme regeneration, Pathway design, In vitro synthetic biology |
url |
http://www.sciencedirect.com/science/article/pii/S2405805X18300255 |
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