An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design

Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and...

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Main Authors: Ting Shi, Pingping Han, Chun You, Yi-Heng P. Job Zhang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2018-09-01
Series:Synthetic and Systems Biotechnology
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X18300255
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spelling doaj-4d227e8c4551426a9bfdab2c027144152021-02-02T08:42:49ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2018-09-0133186195An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway designTing Shi0Pingping Han1Chun You2Yi-Heng P. Job Zhang3Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaCorresponding author.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaCorresponding author..; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaAlthough most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes, has been proposed for low-cost biomanufacturing of bioenergy, food, biochemicals, and nutraceuticals. In addition to the most important advantage-high product yield, in vitro synthetic biology platform features several other biomanufacturing advantages, such as fast reaction rate, easy product separation, open process control, broad reaction condition, tolerance to toxic substrates or products, and so on. In this article, we present the basic bottom-up design principles of in vitro synthetic pathway from basic building blocks-BioBricks (thermoenzymes and/or immobilized enzymes) to building modules (e.g., enzyme complexes or multiple enzymes as a module) with specific functions. With development in thermostable building blocks-BioBricks and modules, the in vitro synthetic biology platform would open a new biomanufacturing age for the cost-competitive production of biocommodities. Keywords: Biomanufacturing, Coenzyme regeneration, Pathway design, In vitro synthetic biologyhttp://www.sciencedirect.com/science/article/pii/S2405805X18300255
collection DOAJ
language English
format Article
sources DOAJ
author Ting Shi
Pingping Han
Chun You
Yi-Heng P. Job Zhang
spellingShingle Ting Shi
Pingping Han
Chun You
Yi-Heng P. Job Zhang
An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
Synthetic and Systems Biotechnology
author_facet Ting Shi
Pingping Han
Chun You
Yi-Heng P. Job Zhang
author_sort Ting Shi
title An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_short An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_full An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_fullStr An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_full_unstemmed An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_sort in vitro synthetic biology platform for emerging industrial biomanufacturing: bottom-up pathway design
publisher KeAi Communications Co., Ltd.
series Synthetic and Systems Biotechnology
issn 2405-805X
publishDate 2018-09-01
description Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes, has been proposed for low-cost biomanufacturing of bioenergy, food, biochemicals, and nutraceuticals. In addition to the most important advantage-high product yield, in vitro synthetic biology platform features several other biomanufacturing advantages, such as fast reaction rate, easy product separation, open process control, broad reaction condition, tolerance to toxic substrates or products, and so on. In this article, we present the basic bottom-up design principles of in vitro synthetic pathway from basic building blocks-BioBricks (thermoenzymes and/or immobilized enzymes) to building modules (e.g., enzyme complexes or multiple enzymes as a module) with specific functions. With development in thermostable building blocks-BioBricks and modules, the in vitro synthetic biology platform would open a new biomanufacturing age for the cost-competitive production of biocommodities. Keywords: Biomanufacturing, Coenzyme regeneration, Pathway design, In vitro synthetic biology
url http://www.sciencedirect.com/science/article/pii/S2405805X18300255
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