Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements

<p>Abstract</p> <p>Background</p> <p>Environmental ozone can rapidly degrade cyanine 5 (Cy5), a fluorescent dye commonly used in microarray gene expression studies. Cyanine 3 (Cy3) is much less affected by atmospheric ozone. Degradation of the Cy5 signal relative to the...

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Bibliographic Details
Main Authors: Scully Adam T, Moland Carrie L, Desai Varsha G, Han Tao, Melvin Cathy D, Branham William S, Fuscoe James C
Format: Article
Language:English
Published: BMC 2007-02-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/7/8