Highly Efficient Genome Engineering in Bacillus anthracis and Bacillus cereus Using the CRISPR/Cas9 System

Highly Efficient Genome Engineering in Bacillus anthracis and Bacillus cereus Using the CRISPR/Cas9 System

Genome editing is an effective tool for the functional examination of bacterial genes and for live attenuated vaccine construction. Here, we report a method to edit the genomic DNA of Bacillus anthracis and Bacillus cereus using the clustered regularly interspaced short palindromic repeats (CRISPR)/...

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Bibliographic Details
Main Authors: Yanchun Wang, Dongshu Wang, Xiaojing Wang, Haoxia Tao, Erling Feng, Li Zhu, Chao Pan, Bowen Wang, Chunjie Liu, Xiankai Liu, Hengliang Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-08-01
Series:Frontiers in Microbiology
Subjects:
CRISPR/Cas9
Bacillus cereus sensu lato group
Bacillus anthracis
Bacillus cereus
large genomic deletion
genomic site-specific mutagenesis
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.01932/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.01932/full

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