Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field
The pulsed electric field (PEF) technology has been widely applied to inactivate pathogenic bacteria in food products. Though irreversible pore formation and membrane disruption is considered to be the main contributing factor to PEF’s sterilizing effects, the exact molecular mechanisms remain poorl...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-11-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02644/full |
id |
doaj-4d9efc6fc1e34cd08a95bd5203631ea3 |
---|---|
record_format |
Article |
spelling |
doaj-4d9efc6fc1e34cd08a95bd5203631ea32020-11-25T02:33:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02644479452Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric FieldZhenyu Liu0Lingying Zhao1Qin Zhang2Nan Huo3Xiaojing Shi4Linwei Li5Liyan Jia6Yuanyuan Lu7Yong Peng8Yanbo Song9Information Science and Engineering College, Shanxi Agricultural University, Jinzhong, ChinaDepartment of Food, Agricultural and Biological Engineering, The Ohio State University, Columbus, OH, United StatesLife Science College, Shanxi Agricultural University, Jinzhong, ChinaLife Science College, Shanxi Agricultural University, Jinzhong, ChinaLife Science College, Shanxi Agricultural University, Jinzhong, ChinaInformation Science and Engineering College, Shanxi Agricultural University, Jinzhong, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Jinzhong, ChinaLife Science College, Shanxi Agricultural University, Jinzhong, ChinaShanghai Applied Protein Technology Co., Ltd., Shanghai, ChinaLife Science College, Shanxi Agricultural University, Jinzhong, ChinaThe pulsed electric field (PEF) technology has been widely applied to inactivate pathogenic bacteria in food products. Though irreversible pore formation and membrane disruption is considered to be the main contributing factor to PEF’s sterilizing effects, the exact molecular mechanisms remain poorly understood. In this study, by using mass spectrometry (MS)-based label-free quantitative proteomic analysis, we compared the protein profiles of PEF-treated and untreated Escherichia coli. We identified a total of 175 differentially expressed proteins, including 52 candidates that were only detected in at least two of the three samples in one experiment group but not in the other group. Functional analysis revealed that the differential proteins were primarily involved in the regulation of cell membrane composition and integrity, stress response, as well as various metabolic processes. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was conducted on the genes of selected differential proteins at varying PEF intensities, which were known to result in different cell killing levels. The qRT-PCR data confirmed that the proteomic results could be reliably used for further data interpretation, and that the changes in the expression levels of the differential candidates were, to a large extent, caused directly by the PEF treatment. The findings of the current study offered valuable insight into PEF-induced cell inactivation.https://www.frontiersin.org/article/10.3389/fmicb.2019.02644/fullpulsed electric fieldE. coliproteomicscell inactivationmolecular mechanisms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhenyu Liu Lingying Zhao Qin Zhang Nan Huo Xiaojing Shi Linwei Li Liyan Jia Yuanyuan Lu Yong Peng Yanbo Song |
spellingShingle |
Zhenyu Liu Lingying Zhao Qin Zhang Nan Huo Xiaojing Shi Linwei Li Liyan Jia Yuanyuan Lu Yong Peng Yanbo Song Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field Frontiers in Microbiology pulsed electric field E. coli proteomics cell inactivation molecular mechanisms |
author_facet |
Zhenyu Liu Lingying Zhao Qin Zhang Nan Huo Xiaojing Shi Linwei Li Liyan Jia Yuanyuan Lu Yong Peng Yanbo Song |
author_sort |
Zhenyu Liu |
title |
Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field |
title_short |
Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field |
title_full |
Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field |
title_fullStr |
Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field |
title_full_unstemmed |
Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field |
title_sort |
proteomics-based mechanistic investigation of escherichia coli inactivation by pulsed electric field |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-11-01 |
description |
The pulsed electric field (PEF) technology has been widely applied to inactivate pathogenic bacteria in food products. Though irreversible pore formation and membrane disruption is considered to be the main contributing factor to PEF’s sterilizing effects, the exact molecular mechanisms remain poorly understood. In this study, by using mass spectrometry (MS)-based label-free quantitative proteomic analysis, we compared the protein profiles of PEF-treated and untreated Escherichia coli. We identified a total of 175 differentially expressed proteins, including 52 candidates that were only detected in at least two of the three samples in one experiment group but not in the other group. Functional analysis revealed that the differential proteins were primarily involved in the regulation of cell membrane composition and integrity, stress response, as well as various metabolic processes. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was conducted on the genes of selected differential proteins at varying PEF intensities, which were known to result in different cell killing levels. The qRT-PCR data confirmed that the proteomic results could be reliably used for further data interpretation, and that the changes in the expression levels of the differential candidates were, to a large extent, caused directly by the PEF treatment. The findings of the current study offered valuable insight into PEF-induced cell inactivation. |
topic |
pulsed electric field E. coli proteomics cell inactivation molecular mechanisms |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02644/full |
work_keys_str_mv |
AT zhenyuliu proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT lingyingzhao proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT qinzhang proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT nanhuo proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT xiaojingshi proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT linweili proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT liyanjia proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT yuanyuanlu proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT yongpeng proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield AT yanbosong proteomicsbasedmechanisticinvestigationofescherichiacoliinactivationbypulsedelectricfield |
_version_ |
1724815932817145856 |