Optimization of coffee (Coffea arabica) transformation parameters using uidA and hpt genes: effect of osmotic pre-treatment, helium pressure and target distance

Optimization of coffee (Coffea arabica) transformation parameters using uidA and hpt genes: effect of osmotic pre-treatment, helium pressure and target distance

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The aim of this work was to optimize the biolistic delivery parameters that affect the DNA delivery and stable expression of marker genes into coffee tissues (Coffea arabica. L. cvs. Caturra and Catuaí). The effect of osmotic preculture length, osmotic concentration of medium, Helium pressure and ta...

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Bibliographic Details
Main Authors: Andrés M Gatica, Griselda Arrieta, Ana M Espinoza
Format: Article
Language:English
Published: Vicerractoría Investigación 2009-11-01
Series:Revista de Biología Tropical
Subjects:
Caturra
Catuaí
biobalística
tratamiento osmótico
uidA
GUSPlus™
bar
hpt
nptII
Costa Rica
Coffea arabica
biolistic delivery
osmotic treatment
Online Access:http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442009000500014

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http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442009000500014

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