Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium

Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium

KRASG12V and BRAFV600E are oncogenic mutations that activate ERK signalling. Here, the authors use single cell analysis in intestinal organoids and show that BRAFV600E activates ERK in all intestinal cell types, while KRASG12V induces ERK activation in only a subset of cells, depending on cell diffe...

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Main Authors: Raphael Brandt, Thomas Sell, Mareen Lüthen, Florian Uhlitz, Bertram Klinger, Pamela Riemer, Claudia Giesecke-Thiel, Silvia Schulze, Ismail Amr El-Shimy, Desiree Kunkel, Beatrix Fauler, Thorsten Mielke, Norbert Mages, Bernhard G. Herrmann, Christine Sers, Nils Blüthgen, Markus Morkel
Format: Article
Language:English
Published: Nature Publishing Group 2019-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-10954-y
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spelling doaj-4dc8feb67d344b21b71aced69388d1f82021-05-11T12:15:35ZengNature Publishing GroupNature Communications2041-17232019-07-0110111510.1038/s41467-019-10954-yCell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epitheliumRaphael Brandt0Thomas Sell1Mareen Lüthen2Florian Uhlitz3Bertram Klinger4Pamela Riemer5Claudia Giesecke-Thiel6Silvia Schulze7Ismail Amr El-Shimy8Desiree Kunkel9Beatrix Fauler10Thorsten Mielke11Norbert Mages12Bernhard G. Herrmann13Christine Sers14Nils Blüthgen15Markus Morkel16Institute of Pathology, Charité Universitätsmedizin BerlinInstitute of Pathology, Charité Universitätsmedizin BerlinInstitute of Pathology, Charité Universitätsmedizin BerlinInstitute of Pathology, Charité Universitätsmedizin BerlinInstitute of Pathology, Charité Universitätsmedizin BerlinInstitute of Pathology, Charité Universitätsmedizin BerlinDepartment of Cell Biology, German Rheumatism Research Center, Leibniz InstituteInstitute of Pathology, Charité Universitätsmedizin BerlinInstitute of Pathology, Charité Universitätsmedizin BerlinBerlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité - Universitätsmedizin BerlinMax Planck Institute for Molecular GeneticsMax Planck Institute for Molecular GeneticsMax Planck Institute for Molecular GeneticsMax Planck Institute for Molecular GeneticsInstitute of Pathology, Charité Universitätsmedizin BerlinInstitute of Pathology, Charité Universitätsmedizin BerlinInstitute of Pathology, Charité Universitätsmedizin BerlinKRASG12V and BRAFV600E are oncogenic mutations that activate ERK signalling. Here, the authors use single cell analysis in intestinal organoids and show that BRAFV600E activates ERK in all intestinal cell types, while KRASG12V induces ERK activation in only a subset of cells, depending on cell differentiation state.https://doi.org/10.1038/s41467-019-10954-y
collection DOAJ
language English
format Article
sources DOAJ
author Raphael Brandt
Thomas Sell
Mareen Lüthen
Florian Uhlitz
Bertram Klinger
Pamela Riemer
Claudia Giesecke-Thiel
Silvia Schulze
Ismail Amr El-Shimy
Desiree Kunkel
Beatrix Fauler
Thorsten Mielke
Norbert Mages
Bernhard G. Herrmann
Christine Sers
Nils Blüthgen
Markus Morkel
spellingShingle Raphael Brandt
Thomas Sell
Mareen Lüthen
Florian Uhlitz
Bertram Klinger
Pamela Riemer
Claudia Giesecke-Thiel
Silvia Schulze
Ismail Amr El-Shimy
Desiree Kunkel
Beatrix Fauler
Thorsten Mielke
Norbert Mages
Bernhard G. Herrmann
Christine Sers
Nils Blüthgen
Markus Morkel
Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium
Nature Communications
author_facet Raphael Brandt
Thomas Sell
Mareen Lüthen
Florian Uhlitz
Bertram Klinger
Pamela Riemer
Claudia Giesecke-Thiel
Silvia Schulze
Ismail Amr El-Shimy
Desiree Kunkel
Beatrix Fauler
Thorsten Mielke
Norbert Mages
Bernhard G. Herrmann
Christine Sers
Nils Blüthgen
Markus Morkel
author_sort Raphael Brandt
title Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium
title_short Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium
title_full Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium
title_fullStr Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium
title_full_unstemmed Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium
title_sort cell type-dependent differential activation of erk by oncogenic kras in colon cancer and intestinal epithelium
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-07-01
description KRASG12V and BRAFV600E are oncogenic mutations that activate ERK signalling. Here, the authors use single cell analysis in intestinal organoids and show that BRAFV600E activates ERK in all intestinal cell types, while KRASG12V induces ERK activation in only a subset of cells, depending on cell differentiation state.
url https://doi.org/10.1038/s41467-019-10954-y
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