Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

CRISPR-Cas9 has been utilized to screen the genome in loss-of-function studies to identify genetic interactions. Here the authors compare catalytically active and dead Cas9 and observe different off-target effects in a screen for essential genes.

Bibliographic Details
Main Authors: Joseph Rosenbluh, Han Xu, William Harrington, Stanley Gill, Xiaoxing Wang, Francisca Vazquez, David E. Root, Aviad Tsherniak, William C. Hahn
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15403

Internet

https://doi.org/10.1038/ncomms15403

Similar Items