Intracellular water exchange for measuring the dry mass, water mass and changes in chemical composition of living cells.

Intracellular water exchange for measuring the dry mass, water mass and changes in chemical composition of living cells.

We present a method for direct non-optical quantification of dry mass, dry density and water mass of single living cells in suspension. Dry mass and dry density are obtained simultaneously by measuring a cell's buoyant mass sequentially in an H2O-based fluid and a D2O-based fluid. Rapid exchang...

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Bibliographic Details
Main Authors: Francisco Feijó Delgado, Nathan Cermak, Vivian C Hecht, Sungmin Son, Yingzhong Li, Scott M Knudsen, Selim Olcum, John M Higgins, Jianzhu Chen, William H Grover, Scott R Manalis
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3699654?pdf=render

Internet

http://europepmc.org/articles/PMC3699654?pdf=render

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