Intracellular water exchange for measuring the dry mass, water mass and changes in chemical composition of living cells.
We present a method for direct non-optical quantification of dry mass, dry density and water mass of single living cells in suspension. Dry mass and dry density are obtained simultaneously by measuring a cell's buoyant mass sequentially in an H2O-based fluid and a D2O-based fluid. Rapid exchang...
Main Authors: | Francisco Feijó Delgado, Nathan Cermak, Vivian C Hecht, Sungmin Son, Yingzhong Li, Scott M Knudsen, Selim Olcum, John M Higgins, Jianzhu Chen, William H Grover, Scott R Manalis |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3699654?pdf=render |
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