Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette

Abstract Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effe...

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Bibliographic Details
Main Authors: Yuanyuan Huang, Lu Li, Shan Xie, Nannan Zhao, Shuangyan Han, Ying Lin, Suiping Zheng
Format: Article
Language:English
Published: Nature Publishing Group 2017-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-08352-9