Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells
Anticancer properties of tyrindoleninone and 6-bromoisatin from Dicathais orbita were tested against physiologically normal primary human granulosa cells (HGC) and reproductive cancer cell lines. Tyrindoleninone reduced cancer cell viability with IC50 values of 39 µM (KGN; a tumour-derived granulosa...
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Online Access: | http://www.mdpi.com/1660-3397/10/1/64/ |
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doaj-4e25bf7ab84a423f9741d1c7d25a6fc82020-11-24T23:05:44ZengMDPI AGMarine Drugs1660-33972012-01-01101648310.3390/md10010064Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa CellsFiona YoungKirsten BenkendorffVicki EdwardsAnticancer properties of tyrindoleninone and 6-bromoisatin from Dicathais orbita were tested against physiologically normal primary human granulosa cells (HGC) and reproductive cancer cell lines. Tyrindoleninone reduced cancer cell viability with IC50 values of 39 µM (KGN; a tumour-derived granulosa cell line), 39 μM (JAr), and 156 μM (OVCAR-3), compared to 3516 μM in HGC. Apoptosis in HGC’s occurred after 4 h at 391 µM tyrindoleninone compared to 20 µM in KGN cells. Differences in apoptosis between HGC and KGN cells were confirmed by TUNEL, with 66 and 31% apoptotic nuclei at 4 h in KGN and HGC, respectively. These marine compounds therefore have potential for development as treatments for female reproductive cancers.http://www.mdpi.com/1660-3397/10/1/64/gynaecological cancersbrominated indolesmarine molluscapoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fiona Young Kirsten Benkendorff Vicki Edwards |
spellingShingle |
Fiona Young Kirsten Benkendorff Vicki Edwards Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells Marine Drugs gynaecological cancers brominated indoles marine mollusc apoptosis |
author_facet |
Fiona Young Kirsten Benkendorff Vicki Edwards |
author_sort |
Fiona Young |
title |
Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells |
title_short |
Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells |
title_full |
Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells |
title_fullStr |
Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells |
title_full_unstemmed |
Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells |
title_sort |
marine compounds selectively induce apoptosis in female reproductive cancer cells but not in primary-derived human reproductive granulosa cells |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2012-01-01 |
description |
Anticancer properties of tyrindoleninone and 6-bromoisatin from Dicathais orbita were tested against physiologically normal primary human granulosa cells (HGC) and reproductive cancer cell lines. Tyrindoleninone reduced cancer cell viability with IC50 values of 39 µM (KGN; a tumour-derived granulosa cell line), 39 μM (JAr), and 156 μM (OVCAR-3), compared to 3516 μM in HGC. Apoptosis in HGC’s occurred after 4 h at 391 µM tyrindoleninone compared to 20 µM in KGN cells. Differences in apoptosis between HGC and KGN cells were confirmed by TUNEL, with 66 and 31% apoptotic nuclei at 4 h in KGN and HGC, respectively. These marine compounds therefore have potential for development as treatments for female reproductive cancers. |
topic |
gynaecological cancers brominated indoles marine mollusc apoptosis |
url |
http://www.mdpi.com/1660-3397/10/1/64/ |
work_keys_str_mv |
AT fionayoung marinecompoundsselectivelyinduceapoptosisinfemalereproductivecancercellsbutnotinprimaryderivedhumanreproductivegranulosacells AT kirstenbenkendorff marinecompoundsselectivelyinduceapoptosisinfemalereproductivecancercellsbutnotinprimaryderivedhumanreproductivegranulosacells AT vickiedwards marinecompoundsselectivelyinduceapoptosisinfemalereproductivecancercellsbutnotinprimaryderivedhumanreproductivegranulosacells |
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