Increasing gene dosage greatly enhances recombinant expression of aquaporins in <it>Pichia pastoris</it>

<p>Abstract</p> <p>Background</p> <p>When performing functional and structural studies, large quantities of pure protein are desired. Most membrane proteins are however not abundantly expressed in their native tissues, which in general rules out purification from natura...

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Bibliographic Details
Main Authors: Kjellbom Per, Alexandersson Erik, Danielson Jonas ÅH, Agemark Maria, Nordén Kristina, Johanson Urban
Format: Article
Language:English
Published: BMC 2011-05-01
Series:BMC Biotechnology
Subjects:
Online Access:http://www.biomedcentral.com/1472-6750/11/47