Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA designs, or Cas proteins, but can also be a platf...

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Bibliographic Details
Main Authors: Jin-Jun Yue, Jin-Ling Yuan, Fu-Hui Wu, Yu-Hsuan Yuan, Qiao-Wei Cheng, Chen-Tran Hsu, Choun-Sea Lin
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-08-01
Series:Frontiers in Genome Editing
Subjects:
RNP
transient transfection
DNA-free
CRISPR/cas (clustered regularly interspaced short palindromic repeats)
protoplasts
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2021.717017/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2021.717017/full

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