Unexpected insertion of carrier DNA sequences into the fission yeast genome during CRISPR–Cas9 mediated gene deletion

Unexpected insertion of carrier DNA sequences into the fission yeast genome during CRISPR–Cas9 mediated gene deletion

Abstract Objectives The fission yeast Schizosaccharomyces pombe is predicted to encode ~ 200 proteins of < 100 amino acids, including a number of previously uncharacterised proteins that are found conserved in related Schizosaccharomyces species only. To begin an investigation of the function of...

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Bibliographic Details
Main Authors: Sophie Longmuir, Nabihah Akhtar, Stuart A. MacNeill
Format: Article
Language:English
Published: BMC 2019-03-01
Series:BMC Research Notes
Subjects:
Microprotein
Fission yeast
Schizosaccharomyces pombe
Oncorhynchus keta
CRISPR–Cas9
Online Access:http://link.springer.com/article/10.1186/s13104-019-4228-x

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http://link.springer.com/article/10.1186/s13104-019-4228-x

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