Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system

Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-ba...

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Bibliographic Details
Main Authors: A.M. Fernandes, P.A.N. Marinho, R.C. Sartore, B.S. Paulsen, R.M. Mariante, L.R. Castilho, S.K. Rehen
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2009-06-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600007