RNA-guided retargeting of Sleeping Beauty transposition in human cells

RNA-guided retargeting of Sleeping Beauty transposition in human cells

An ideal tool for gene therapy would enable efficient gene integration at predetermined sites in the human genome. Here we demonstrate biased genome-wide integration of the Sleeping Beauty (SB) transposon by combining it with components of the CRISPR/Cas9 system. We provide proof-of-concept that it...

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Bibliographic Details
Main Authors: Adrian Kovač, Csaba Miskey, Michael Menzel, Esther Grueso, Andreas Gogol-Döring, Zoltán Ivics
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-03-01
Series:eLife
Subjects:
genetic engineering
CRISPR/Cas
gene targeting
DNA-binding
gene insertion
Online Access:https://elifesciences.org/articles/53868

Internet

https://elifesciences.org/articles/53868

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