Asymmetric PCR for good quality ssDNA generation towards DNA aptamer production

Asymmetric PCR for good quality ssDNA generation towards DNA aptamer production

Aptamers are ssDNA or RNA that binds to wide variety of target molecules with high affinity and specificity producedby systematic evolution of ligands by exponential enrichment (SELEX). Compared to RNA aptamer, DNA aptamer is muchmore stable, favourable to be used in many applications. The most crit...

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Bibliographic Details
Main Authors: Junji Tominaga4, Rajan Saini, Chee-Hock Hoe1, Thean-Hock Tang1, Soo-Choon Tan, Marimuthu Citartan1, Subash C.B. Gopinath4
Format: Article
Language:English
Published: Prince of Songkla University 2012-04-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
optimization
asymmetric PCR
single-stranded DNA
Aptamer
SELEX
Online Access:http://rdo.psu.ac.th/sjstweb/journal/34-2/0125-3395-volume-125-131.pdf

Internet

http://rdo.psu.ac.th/sjstweb/journal/34-2/0125-3395-volume-125-131.pdf

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