SMURF-seq: efficient copy number profiling on long-read sequencers

SMURF-seq: efficient copy number profiling on long-read sequencers

Abstract We present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules. Applying SMURF-seq using the Oxford Nanopore MinION yields up to 30 fragments per read, providing an average of 6.2 and up to 7.5 million m...

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Bibliographic Details
Main Authors: Rishvanth K. Prabakar, Liya Xu, James Hicks, Andrew D. Smith
Format: Article
Language:English
Published: BMC 2019-07-01
Series:Genome Biology
Subjects:
Long-read sequencing
Nanopore sequencing
Copy number variation
Read-counting applications
Online Access:http://link.springer.com/article/10.1186/s13059-019-1732-1

Internet

http://link.springer.com/article/10.1186/s13059-019-1732-1

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