Pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogates
The pyridinium-2-carbaldoximes with quinolinium carboxamide moiety were designed and synthesised as cholinesterase reactivators. The prepared compounds showed intermediate-to-high inhibition of both cholinesterases when compared to standard oximes. Their reactivation ability was evaluated in vitro o...
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2021-01-01
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doaj-4f1eaa9e04f94ca384b62f9d55e4a6562021-01-26T12:13:33ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742021-01-0136143744910.1080/14756366.2020.18699541869954Pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogatesHyun Myung Lee0Rudolf Andrys1Jakub Jonczyk2Kyuneun Kim3Avinash G. Vishakantegowda4David Malinak5Adam Skarka6Monika Schmidt7Michaela Vaskova8Kamil Latka9Marek Bajda10Young-Sik Jung11Barbara Malawska12Kamil Musilek13Division of Bio and Drug Discovery, Korea Research Institute of Chemical TechnologyDepartment of Chemistry, Faculty of Science, University of Hradec KraloveDepartment of Physicochemical Drug Analysis, Faculty of Pharmacy, Jagiellonian University Medical CollegeDivision of Bio and Drug Discovery, Korea Research Institute of Chemical TechnologyDivision of Bio and Drug Discovery, Korea Research Institute of Chemical TechnologyDepartment of Chemistry, Faculty of Science, University of Hradec KraloveDepartment of Chemistry, Faculty of Science, University of Hradec KraloveDepartment of Chemistry, Faculty of Science, University of Hradec KraloveDepartment of Chemistry, Faculty of Science, University of Hradec KraloveDepartment of Physicochemical Drug Analysis, Faculty of Pharmacy, Jagiellonian University Medical CollegeDepartment of Physicochemical Drug Analysis, Faculty of Pharmacy, Jagiellonian University Medical CollegeDivision of Bio and Drug Discovery, Korea Research Institute of Chemical TechnologyDepartment of Physicochemical Drug Analysis, Faculty of Pharmacy, Jagiellonian University Medical CollegeDepartment of Chemistry, Faculty of Science, University of Hradec KraloveThe pyridinium-2-carbaldoximes with quinolinium carboxamide moiety were designed and synthesised as cholinesterase reactivators. The prepared compounds showed intermediate-to-high inhibition of both cholinesterases when compared to standard oximes. Their reactivation ability was evaluated in vitro on human recombinant acetylcholinesterase (hrAChE) and human recombinant butyrylcholinesterase (hrBChE) inhibited by nerve agent surrogates (NIMP, NEMP, and NEDPA) or paraoxon. In the reactivation screening, one compound was able to reactivate hrAChE inhibited by all used organophosphates and two novel compounds were able to reactivate NIMP/NEMP-hrBChE. The reactivation kinetics revealed compound 11 that proved to be excellent reactivator of paraoxon-hrAChE better to obidoxime and showed increased reactivation of NIMP/NEMP-hrBChE, although worse to obidoxime. The molecular interactions of studied reactivators were further identified by in silico calculations. Molecular modelling results revealed the importance of creation of the pre-reactivation complex that could lead to better reactivation of both cholinesterases together with reducing particular interactions for lower intrinsic inhibition by the oxime.http://dx.doi.org/10.1080/14756366.2020.1869954organophosphateacetylcholinesterasebutyrylcholinesterasereactivatoroxime |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyun Myung Lee Rudolf Andrys Jakub Jonczyk Kyuneun Kim Avinash G. Vishakantegowda David Malinak Adam Skarka Monika Schmidt Michaela Vaskova Kamil Latka Marek Bajda Young-Sik Jung Barbara Malawska Kamil Musilek |
spellingShingle |
Hyun Myung Lee Rudolf Andrys Jakub Jonczyk Kyuneun Kim Avinash G. Vishakantegowda David Malinak Adam Skarka Monika Schmidt Michaela Vaskova Kamil Latka Marek Bajda Young-Sik Jung Barbara Malawska Kamil Musilek Pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogates Journal of Enzyme Inhibition and Medicinal Chemistry organophosphate acetylcholinesterase butyrylcholinesterase reactivator oxime |
author_facet |
Hyun Myung Lee Rudolf Andrys Jakub Jonczyk Kyuneun Kim Avinash G. Vishakantegowda David Malinak Adam Skarka Monika Schmidt Michaela Vaskova Kamil Latka Marek Bajda Young-Sik Jung Barbara Malawska Kamil Musilek |
author_sort |
Hyun Myung Lee |
title |
Pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogates |
title_short |
Pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogates |
title_full |
Pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogates |
title_fullStr |
Pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogates |
title_full_unstemmed |
Pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogates |
title_sort |
pyridinium-2-carbaldoximes with quinolinium carboxamide moiety are simultaneous reactivators of acetylcholinesterase and butyrylcholinesterase inhibited by nerve agent surrogates |
publisher |
Taylor & Francis Group |
series |
Journal of Enzyme Inhibition and Medicinal Chemistry |
issn |
1475-6366 1475-6374 |
publishDate |
2021-01-01 |
description |
The pyridinium-2-carbaldoximes with quinolinium carboxamide moiety were designed and synthesised as cholinesterase reactivators. The prepared compounds showed intermediate-to-high inhibition of both cholinesterases when compared to standard oximes. Their reactivation ability was evaluated in vitro on human recombinant acetylcholinesterase (hrAChE) and human recombinant butyrylcholinesterase (hrBChE) inhibited by nerve agent surrogates (NIMP, NEMP, and NEDPA) or paraoxon. In the reactivation screening, one compound was able to reactivate hrAChE inhibited by all used organophosphates and two novel compounds were able to reactivate NIMP/NEMP-hrBChE. The reactivation kinetics revealed compound 11 that proved to be excellent reactivator of paraoxon-hrAChE better to obidoxime and showed increased reactivation of NIMP/NEMP-hrBChE, although worse to obidoxime. The molecular interactions of studied reactivators were further identified by in silico calculations. Molecular modelling results revealed the importance of creation of the pre-reactivation complex that could lead to better reactivation of both cholinesterases together with reducing particular interactions for lower intrinsic inhibition by the oxime. |
topic |
organophosphate acetylcholinesterase butyrylcholinesterase reactivator oxime |
url |
http://dx.doi.org/10.1080/14756366.2020.1869954 |
work_keys_str_mv |
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