Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells

Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells

Abstract Ammonia has a cytotoxic effect and can therefore be used as a selection agent for enrichment of zone I hepatocytes. However, it has not yet been determined whether ammonia-treated hepatocyte-like cells are able to proliferate in vitro. In this study, we employed an ammonia selection strateg...

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Bibliographic Details
Main Authors: Ruri Tsuneishi, Noriaki Saku, Shoko Miyata, Saeko Akiyama, Palaksha Kanive Javaregowda, Kenta Ite, Nagisa Takashima, Masashi Toyoda, Tohru Kimura, Masahiko Kuroda, Atsuko Nakazawa, Mureo Kasahara, Hidenori Nonaka, Akihide Kamiya, Tohru Kiyono, Junji Yamauchi, Akihiro Umezawa
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-90708-3

Internet

https://doi.org/10.1038/s41598-021-90708-3

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