Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers

Abstract The structure of the p7 viroporin, an oligomeric membrane protein ion channel involved in the assembly and release of the hepatitis C virus, was determined from proteins expressed and inserted directly into supported model lipid membranes using cell-free protein expression. Cell-free protei...

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Bibliographic Details
Main Authors: Thomas Soranzo, Donald K. Martin, Jean-Luc Lenormand, Erik B. Watkins
Format: Article
Language:English
Published: Nature Publishing Group 2017-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-03472-8