Primers for Low-Copy Nuclear Genes in the Hawaiian Endemic <i>Clermontia</i> (Campanulaceae) and Cross-Amplification in Lobelioideae

Primers for Low-Copy Nuclear Genes in the Hawaiian Endemic <i>Clermontia</i> (Campanulaceae) and Cross-Amplification in Lobelioideae

Premise of the study: Primers were developed to amplify 12 intron-less, low-copy nuclear genes in the Hawaiian genus <i>Clermontia</i> (Campanulaceae), a suspected tetraploid. Methods and Results: Data from a pooled 454 titanium run of the partial transcriptomes of seven <i>Cler...

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Bibliographic Details
Main Authors: Yohan Pillon, Jennifer Johansen, Tomoko Sakishima, Srikar Chamala, W. Brad Barbazuk, Elizabeth A. Stacy
Format: Article
Language:English
Published: Wiley 2013-06-01
Series:Applications in Plant Sciences
Subjects:
Clermontia
Hawai‘i
Lobelioideae
low-copy nuclear genes
next-generation sequencing
polyploidy
Online Access:http://www.bioone.org/doi/full/10.3732/apps.1200450

Internet

http://www.bioone.org/doi/full/10.3732/apps.1200450

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