A simplified <it>in vitro </it>ligation approach to clone an <it>E1B55k</it>-deleted double-targeted conditionally-replicative adenovirus
<p>Abstract</p> <p>Background</p> <p>Construction of conditionally-replicative Adenovirus (CRAd) is complex and time-consuming. While homologous recombination (HR) using a two-plasmid system in bacteria is commonly used to generate CRAds, alternative methods may be requ...
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| Format: | Article |
| Language: | English |
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BMC
2009-02-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/6/1/18 |