Detection of SARS-CoV-2 RNA by multiplex RT-qPCR.

Detection of SARS-CoV-2 RNA by multiplex RT-qPCR.

The current quantitative reverse transcription PCR (RT-qPCR) assay recommended for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing in the United States requires analysis of 3 genomic targets per sample: 2 viral and 1 host. To simplify testing and reduce the volume of required re...

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Bibliographic Details
Main Authors: Eriko Kudo, Benjamin Israelow, Chantal B F Vogels, Peiwen Lu, Anne L Wyllie, Maria Tokuyama, Arvind Venkataraman, Doug E Brackney, Isabel M Ott, Mary E Petrone, Rebecca Earnest, Sarah Lapidus, M Catherine Muenker, Adam J Moore, Arnau Casanovas-Massana, Yale IMPACT Research Team, Saad B Omer, Charles S Dela Cruz, Shelli F Farhadian, Albert I Ko, Nathan D Grubaugh, Akiko Iwasaki
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-10-01
Series:PLoS Biology
Online Access:https://doi.org/10.1371/journal.pbio.3000867

Internet

https://doi.org/10.1371/journal.pbio.3000867

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