Evaluation of four viroid RNA extraction methods for the molecular diagnosis of CEVd in Citrus lemon using RT-PCR, Dot blot and Northern blot
An efficient method for RNA extraction that leads to RNA high yield and purity is a technical issue relevant for development and optimization of molecular diagnostic methods aimed to detect viroid infections in citrus varieties. Residual contaminants may affect RNA detection depending on the molecul...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elfos Scientiae
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| Series: | Biotecnología Aplicada |
| Subjects: | |
| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522013000200006&lng=en&tlng=en |