Effects of intraventricular methotrexate administration on Cuprizone-induced demyelination in mice
We previously showed that intrathecal administration of methotrexate slowed disability progression in multiple sclerosis patients with progressive disease. In general MS patients with progressive disease respond poorly to anti-inflammatory therapies. In order to better understand the mechanism by wh...
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2013-10-01
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doaj-5065821b3c524cb7aec6064292bc4e782020-11-24T20:58:00ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992013-10-01610.3389/fnmol.2013.0003458269Effects of intraventricular methotrexate administration on Cuprizone-induced demyelination in miceAndre Michael Mueller0Adam eNassery1Hana eConlon2Xinhe eLiu3Massimiliano eCristofanilli4Esther eJun5Bo Hyung eYoon6Saud Ahmed Sadiq7Tisch MS Research Center of New YorkAlbert Einstein College of MedicineColumbia University School of NursingTisch MS Research Center of New YorkTisch MS Research Center of New YorkUniversity of California, IrvineTisch MS Research Center of New YorkTisch MS Research Center of New YorkWe previously showed that intrathecal administration of methotrexate slowed disability progression in multiple sclerosis patients with progressive disease. In general MS patients with progressive disease respond poorly to anti-inflammatory therapies. In order to better understand the mechanism by which methotrexate is protective in progressive MS, we analyzed its impact on the non-inflammatory cuprizone-induced demyelination model.When low-dose methotrexate was administered intracerebroventricularly it reduced demyelination and accumulation of GFAP+ reactive astrocytes in the corpus callosum. Administration of methotrexate after the withdrawal of cuprizone neither delayed remyelination nor influenced the number of astrocytes in the corpus callosum suggesting that methotrexate does not interfere with repair processes in the CNS. Moreover, methotrexate increased the expression of IGF1 in vitro and in vivo, a factor known to protect oligodendrocytes and limit the activation of astrocytes. Our studies show that methotrexate has an impact on pathogenic process in a demyelination model whose pathophysiological basis is not primarily related to inflammatory mechanisms, similar to neurodegenerative mechanisms associated with progressive MS. The pronounced inhibitory influence of methotrexate on the accumulation of astrocytes in the corpus callosum suggests that intrathecal methotrexate modulates astroglial activation in progressive MS possibly by promoting CNS production of IGF1.http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00034/fullAstrocytesMethotrexateMultiple SclerosisEAEIGF1demyelination |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andre Michael Mueller Adam eNassery Hana eConlon Xinhe eLiu Massimiliano eCristofanilli Esther eJun Bo Hyung eYoon Saud Ahmed Sadiq |
spellingShingle |
Andre Michael Mueller Adam eNassery Hana eConlon Xinhe eLiu Massimiliano eCristofanilli Esther eJun Bo Hyung eYoon Saud Ahmed Sadiq Effects of intraventricular methotrexate administration on Cuprizone-induced demyelination in mice Frontiers in Molecular Neuroscience Astrocytes Methotrexate Multiple Sclerosis EAE IGF1 demyelination |
author_facet |
Andre Michael Mueller Adam eNassery Hana eConlon Xinhe eLiu Massimiliano eCristofanilli Esther eJun Bo Hyung eYoon Saud Ahmed Sadiq |
author_sort |
Andre Michael Mueller |
title |
Effects of intraventricular methotrexate administration on Cuprizone-induced demyelination in mice |
title_short |
Effects of intraventricular methotrexate administration on Cuprizone-induced demyelination in mice |
title_full |
Effects of intraventricular methotrexate administration on Cuprizone-induced demyelination in mice |
title_fullStr |
Effects of intraventricular methotrexate administration on Cuprizone-induced demyelination in mice |
title_full_unstemmed |
Effects of intraventricular methotrexate administration on Cuprizone-induced demyelination in mice |
title_sort |
effects of intraventricular methotrexate administration on cuprizone-induced demyelination in mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Neuroscience |
issn |
1662-5099 |
publishDate |
2013-10-01 |
description |
We previously showed that intrathecal administration of methotrexate slowed disability progression in multiple sclerosis patients with progressive disease. In general MS patients with progressive disease respond poorly to anti-inflammatory therapies. In order to better understand the mechanism by which methotrexate is protective in progressive MS, we analyzed its impact on the non-inflammatory cuprizone-induced demyelination model.When low-dose methotrexate was administered intracerebroventricularly it reduced demyelination and accumulation of GFAP+ reactive astrocytes in the corpus callosum. Administration of methotrexate after the withdrawal of cuprizone neither delayed remyelination nor influenced the number of astrocytes in the corpus callosum suggesting that methotrexate does not interfere with repair processes in the CNS. Moreover, methotrexate increased the expression of IGF1 in vitro and in vivo, a factor known to protect oligodendrocytes and limit the activation of astrocytes. Our studies show that methotrexate has an impact on pathogenic process in a demyelination model whose pathophysiological basis is not primarily related to inflammatory mechanisms, similar to neurodegenerative mechanisms associated with progressive MS. The pronounced inhibitory influence of methotrexate on the accumulation of astrocytes in the corpus callosum suggests that intrathecal methotrexate modulates astroglial activation in progressive MS possibly by promoting CNS production of IGF1. |
topic |
Astrocytes Methotrexate Multiple Sclerosis EAE IGF1 demyelination |
url |
http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00034/full |
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