Glucuronoyl Esterase Screening and Characterization Assays Utilizing Commercially Available Benzyl Glucuronic Acid Ester

Glucuronoyl Esterase Screening and Characterization Assays Utilizing Commercially Available Benzyl Glucuronic Acid Ester

Research on glucuronoyl esterases (GEs) has been hampered by the lack of enzyme assays based on easily obtainable substrates. While benzyl d-glucuronic acid ester (BnGlcA) is a commercially available substrate that can be used for GE assays, several considerations regarding substrate instability, li...

Full description

Bibliographic Details
Main Authors: Hampus Sunner, Maria-Despoina Charavgi, Lisbeth Olsson, Evangelos Topakas, Paul Christakopoulos
Format: Article
Language:English
Published: MDPI AG 2015-09-01
Series:Molecules
Subjects:
glucuronic acid
glucuronoyl esterase
enzymatic assay
benzyl glucuronic acid ester
enzyme kinetics
Michaelis-Menten parameter estimation
Online Access:http://www.mdpi.com/1420-3049/20/10/17807

Internet

http://www.mdpi.com/1420-3049/20/10/17807

Similar Items