Selection of Reference Genes for Quantitative Real-Time PCR in Aquatica leii (Coleoptera: Lampyridae) Under Five Different Experimental Conditions

Selection of Reference Genes for Quantitative Real-Time PCR in Aquatica leii (Coleoptera: Lampyridae) Under Five Different Experimental Conditions

Aquatic fireflies are important indicators of the quality of freshwater environments and key models for research on insect adaptation to freshwater environments. For these investigations, gene expression analyses using quantitative real-time PCR are heavily dependent on reliable reference genes. In...

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Bibliographic Details
Main Authors: Xiao-Jie Yang, Hai-Long Zheng, Ying-Yang Liu, Hong-Wei Li, Yu-Hang Jiang, Lian-Bing Lin, Xian-Yu Deng, Qi-Lin Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Physiology
Subjects:
Aquatica leii
qPCR
reference gene
gene expression
stability evaluation
Online Access:https://www.frontiersin.org/article/10.3389/fphys.2020.555233/full

Internet

https://www.frontiersin.org/article/10.3389/fphys.2020.555233/full

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