Real-time isothermal nucleic acid amplification detection in resource-limited settings: a description of an open-source miniature fluorimeter

Background: The detection and quantification of nucleic acids traditionally relies on PCR, which requires the use of expensive thermocyclers with integrated fluorescence detection of amplicons. While isothermal nucleic acid amplification technologies eliminate the need for thermocycling, fluorescenc...

Full description

Bibliographic Details
Main Authors: Jackson Coole, BS, Alex Kortum, MSc, Yubo Tang, PhD, Imran Vohra, BS, Mary Natoli, PhD, Kathryn Kundrod, PhD, Rebecca Richards-Kortum, PhD
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:The Lancet Global Health
Online Access:http://www.sciencedirect.com/science/article/pii/S2214109X21001145