Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication.

5-Bromo-2'-deoxyuridine (BrdU) labelling and immunostaining is commonly used for the detection of DNA replication using specific antibodies. Previously, we found that these antibodies significantly differ in their affinity to BrdU. Our present data showed that one of the reasons for the differe...

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Bibliographic Details
Main Authors: Anna Ligasová, Petr Konečný, Ivo Frydrych, Karel Koberna
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5373633?pdf=render