A new strategy for seamless gene editing and marker recycling in Saccharomyces cerevisiae using lethal effect of Cwp1

Abstract Technologies development for seamless gene editing and marker recycling has allowed frequent genomic engineering in Saccharomyces cerevisiae for desired laboratory strains and cell factory. Alternative new approaches are still required for complicated scenarios. In this study, we report tha...

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Bibliographic Details
Main Authors: Yuxiao Hu, Yanrong Jia, Xiangdong Zhao, Zihao Yang, Zhimin Hao, Jingao Dong, Fanli Zeng
Format: Article
Language:English
Published: Wiley 2019-06-01
Series:MicrobiologyOpen
Subjects:
Online Access:https://doi.org/10.1002/mbo3.750