Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays.

Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays.

Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infection. The development of automated DNA extraction methodologies and PCR systems is an important step toward the standardization of protocols in routine diagnosis. To date, there are only two commerci...

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Bibliographic Details
Main Authors: Alba Abras, Cristina Ballart, Teresa Llovet, Carme Roig, Cristina Gutiérrez, Silvia Tebar, Pere Berenguer, María-Jesús Pinazo, Elizabeth Posada, Joaquim Gascón, Alejandro G Schijman, Montserrat Gállego, Carmen Muñoz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5903661?pdf=render

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http://europepmc.org/articles/PMC5903661?pdf=render

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