SSR-Patchwork: An Optimized Protocol to Obtain a Rapid and Inexpensive SSR Library Using First-Generation Sequencing Technology

SSR-Patchwork: An Optimized Protocol to Obtain a Rapid and Inexpensive SSR Library Using First-Generation Sequencing Technology

Premise of the study: We have optimized a version of a microsatellite loci isolation protocol for first-generation sequencing (FGS) technologies. The protocol is optimized to reduce the cost and number of steps, and it combines some procedures from previous simple sequence repeat (SSR) protocols wit...

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Bibliographic Details
Main Authors: Antonietta Di Maio, Olga De Castro
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Applications in Plant Sciences
Subjects:
double PCR enrichment
first-generation sequencing
microsatellites
optimized protocol
patchwork
SSR library
Online Access:http://www.bioone.org/doi/full/10.3732/apps.1200158

Internet

http://www.bioone.org/doi/full/10.3732/apps.1200158

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