Identification and validation of a Schistosoma japonicum U6 promoter

Identification and validation of a Schistosoma japonicum U6 promoter

Abstract Background RNA polymerase III promoters have been widely used to express short hairpin-RNA (shRNA), microRNA (miRNA), and small guide RNA (sgRNA) in gene functional analysis in a variety of organisms including Schistosoma mansoni. However, no endogenous RNA polymerase III promoters have bee...

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Bibliographic Details
Main Authors: Qing Li, Wan Wang, Nan Zhao, Pengcheng Li, Yue Xin, Wei Hu
Format: Article
Language:English
Published: BMC 2017-06-01
Series:Parasites & Vectors
Subjects:
Schistosoma japonicum
U6 promoter
sgRNA
RNAi
Lentivirus
Cas9 nuclease
Online Access:http://link.springer.com/article/10.1186/s13071-017-2207-4

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http://link.springer.com/article/10.1186/s13071-017-2207-4

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