Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout, Oncorhynchus mykiss

A novel method for the establishment and long-term maintenance of ex vivo cultures from intestinal regions of the rainbow trout, Oncorhynchus mykiss (Walbaum), is reported. Adherence of cells was observed within hours, epithelial island formation recorded at 48 h and rapid proliferation with conflue...

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Bibliographic Details
Main Authors: Laura M. Langan, Stewart F. Owen, Awadhesh N. Jha
Format: Article
Language:English
Published: The Company of Biologists 2018-03-01
Series:Biology Open
Subjects:
3Rs
Online Access:http://bio.biologists.org/content/7/3/bio032870