A synthetic peptide library for benchmarking crosslinking-mass spectrometry search engines for proteins and protein complexes

Validating crosslinking-mass spectrometry workflows is hampered by the lack of a ground truth to assess the robustness of the crosslink identifications. Here, the authors present a synthetic library of crosslinked peptides, enabling unambiguous discrimination of correct and incorrect crosslink ident...

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Bibliographic Details
Main Authors: Rebecca Beveridge, Johannes Stadlmann, Josef M. Penninger, Karl Mechtler
Format: Article
Language:English
Published: Nature Publishing Group 2020-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-14608-2