Mos1 Element-Mediated CRISPR Integration of Transgenes in Caenorhabditis elegans

The introduction of exogenous genes in single-copy at precise genomic locations is a powerful tool that has been widely used in the model organism Caenorhabditis elegans. Here, we have streamlined the process by creating a rapid, cloning-free method of single-copy transgene insertion we call Mos1 el...

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Bibliographic Details
Main Authors: Nicholas S. Philip, Fernando Escobedo, Laura L. Bahr, Brandon J. Berry, Andrew P. Wojtovich
Format: Article
Language:English
Published: Oxford University Press 2019-08-01
Series:G3: Genes, Genomes, Genetics
Subjects:
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.119.400399