Mos1 Element-Mediated CRISPR Integration of Transgenes in Caenorhabditis elegans
The introduction of exogenous genes in single-copy at precise genomic locations is a powerful tool that has been widely used in the model organism Caenorhabditis elegans. Here, we have streamlined the process by creating a rapid, cloning-free method of single-copy transgene insertion we call Mos1 el...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Oxford University Press
2019-08-01
|
Series: | G3: Genes, Genomes, Genetics |
Subjects: | |
Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.119.400399 |