Design parameters to control synthetic gene expression in Escherichia coli.

BACKGROUND:Production of proteins as therapeutic agents, research reagents and molecular tools frequently depends on expression in heterologous hosts. Synthetic genes are increasingly used for protein production because sequence information is easier to obtain than the corresponding physical DNA. Pr...

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Bibliographic Details
Main Authors: Mark Welch, Sridhar Govindarajan, Jon E Ness, Alan Villalobos, Austin Gurney, Jeremy Minshull, Claes Gustafsson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-09-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2736378?pdf=render