CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish

CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish

Abstract Background Gene targeting by homology-directed repair (HDR) can precisely edit the genome and is a versatile tool for biomedical research. However, the efficiency of HDR-based modification is still low in many model organisms including zebrafish. Recently, long single-stranded DNA (lssDNA)...

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Bibliographic Details
Main Authors: Haipeng Bai, Lijun Liu, Ke An, Xiaochan Lu, Michael Harrison, Yanqiu Zhao, Ruibin Yan, Zhijie Lu, Song Li, Shuo Lin, Fang Liang, Wei Qin
Format: Article
Language:English
Published: BMC 2020-01-01
Series:BMC Genomics
Subjects:
CRISPR/Cas9
Homology-directed repair
Long single-stranded DNA
Next-generation sequencing
Disease modeling
Genome editing
Online Access:https://doi.org/10.1186/s12864-020-6493-4

Internet

https://doi.org/10.1186/s12864-020-6493-4

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