Re-sensitization of Mycobacterium smegmatis to Rifampicin Using CRISPR Interference Demonstrates Its Utility for the Study of Non-essential Drug Resistance Traits

Re-sensitization of Mycobacterium smegmatis to Rifampicin Using CRISPR Interference Demonstrates Its Utility for the Study of Non-essential Drug Resistance Traits

A greater understanding of the genes involved in antibiotic resistance in Mycobacterium tuberculosis (Mtb) is necessary for the design of improved therapies. Clustered regularly interspaced short palindromic repeat interference (CRISPRi) has been previously utilized in mycobacteria to identify novel...

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Bibliographic Details
Main Authors: Valwynne Faulkner, Adrienne Adele Cox, Shan Goh, Annelies van Bohemen, Amanda J. Gibson, Oliver Liebster, Brendan W. Wren, Sam Willcocks, Sharon L. Kendall
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Microbiology
Subjects:
antibiotic resistance
tuberculosis
CRISPRi
mycobacteria
rifampicin resistance
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.619427/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2020.619427/full

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