An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells.
Ebola virus (EBOV), a member of the mononegaviral family Filoviridae, causes severe disease associated with high lethality in humans. Despite enormous progress in development of EBOV medical countermeasures, no anti-EBOV treatment has been approved. We designed an immunotoxin in which a single-chain...
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2021-01-01
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doaj-520cc08e80134a52a681afdaa509a0012021-05-14T04:30:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01161e024502410.1371/journal.pone.0245024An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells.Yingyun CaiShuiqing YuXiaoli ChiSheli R RadoshitzkyJens H KuhnEdward A BergerEbola virus (EBOV), a member of the mononegaviral family Filoviridae, causes severe disease associated with high lethality in humans. Despite enormous progress in development of EBOV medical countermeasures, no anti-EBOV treatment has been approved. We designed an immunotoxin in which a single-chain variable region fragment of the EBOV glycoprotein-specific monoclonal antibody 6D8 was fused to the effector domains of Pseudomonas aeruginosa exotoxin A (PE38). This immunotoxin, 6D8-PE38, bound specifically to cells expressing EBOV glycoproteins. Importantly, 6D8-PE38 targeted EBOV-infected cells, as evidenced by inhibition of infectious EBOV production from infected cells, including primary human macrophages. The data presented here provide a proof of concept for immunotoxin-based targeted killing of infected cells as a potential antiviral intervention for Ebola virus disease.https://doi.org/10.1371/journal.pone.0245024 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yingyun Cai Shuiqing Yu Xiaoli Chi Sheli R Radoshitzky Jens H Kuhn Edward A Berger |
spellingShingle |
Yingyun Cai Shuiqing Yu Xiaoli Chi Sheli R Radoshitzky Jens H Kuhn Edward A Berger An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells. PLoS ONE |
author_facet |
Yingyun Cai Shuiqing Yu Xiaoli Chi Sheli R Radoshitzky Jens H Kuhn Edward A Berger |
author_sort |
Yingyun Cai |
title |
An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells. |
title_short |
An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells. |
title_full |
An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells. |
title_fullStr |
An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells. |
title_full_unstemmed |
An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells. |
title_sort |
immunotoxin targeting ebola virus glycoprotein inhibits ebola virus production from infected cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2021-01-01 |
description |
Ebola virus (EBOV), a member of the mononegaviral family Filoviridae, causes severe disease associated with high lethality in humans. Despite enormous progress in development of EBOV medical countermeasures, no anti-EBOV treatment has been approved. We designed an immunotoxin in which a single-chain variable region fragment of the EBOV glycoprotein-specific monoclonal antibody 6D8 was fused to the effector domains of Pseudomonas aeruginosa exotoxin A (PE38). This immunotoxin, 6D8-PE38, bound specifically to cells expressing EBOV glycoproteins. Importantly, 6D8-PE38 targeted EBOV-infected cells, as evidenced by inhibition of infectious EBOV production from infected cells, including primary human macrophages. The data presented here provide a proof of concept for immunotoxin-based targeted killing of infected cells as a potential antiviral intervention for Ebola virus disease. |
url |
https://doi.org/10.1371/journal.pone.0245024 |
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