Susceptibility of aging mice to listeriosis: Role of anti-inflammatory responses with enhanced Treg-cell expression of CD39/CD73 and Th-17 cells
Foodborne Listeria monocytogenes (Lm) causes serious illness and death in immunosuppressed hosts, including the elderly population. We investigated Lm susceptibility and inflammatory cytokines in geriatric mice. Young-adult and old mice were gavaged with a Lm strain Lmo-InlAm. Tissues were assayed f...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2020-02-01
|
Series: | International Journal of Medical Microbiology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1438422120300072 |
id |
doaj-52147186b78244adb77b7835e81aac64 |
---|---|
record_format |
Article |
spelling |
doaj-52147186b78244adb77b7835e81aac642021-06-05T06:03:54ZengElsevierInternational Journal of Medical Microbiology1438-42212020-02-013102151397Susceptibility of aging mice to listeriosis: Role of anti-inflammatory responses with enhanced Treg-cell expression of CD39/CD73 and Th-17 cellsM. Samiul Alam0Christopher Cavanaugh1Marion Pereira2Uma Babu3Kristina Williams4Corresponding author at: Center for Food Safety and Applied Nutrition, US FDA, Laurel, MD, 20708, USA.; Immunobiology Branch, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, 20708, USAImmunobiology Branch, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, 20708, USAImmunobiology Branch, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, 20708, USAImmunobiology Branch, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, 20708, USAImmunobiology Branch, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, 20708, USAFoodborne Listeria monocytogenes (Lm) causes serious illness and death in immunosuppressed hosts, including the elderly population. We investigated Lm susceptibility and inflammatory cytokines in geriatric mice. Young-adult and old mice were gavaged with a Lm strain Lmo-InlAm. Tissues were assayed for Lm burden and splenocytes were analyzed for Th1/Th2/Th17/Treg responses and expression of CD39 and CD73. Old Lm-infected mice lost body-weight dose-dependently, had higher Lm colonization, and showed higher inflammatory responses than Lm-infected young-adult mice. After infection, IL-17 levels increased significantly in old mice whereas IFN-γ levels were unchanged. Levels of IL-10 and Treg cells were increased in infected old mice as compared to infected young-adult mice. Age-dependent enhanced expression of CD39/CD73 was observed in purified Treg prior to infection, suggesting increased baseline adenosine production in old mice. Lm lysate-treated splenocytes from older mice produced significantly higher levels of IL-10, IL17, and IL-1β, produced less IFN-γ and IL-2, and proliferated less than splenocytes from young-adult mice. Data suggests that older mice maybe more susceptible to Lm infection due to an imbalance of Th cell responses with disproportionate and persistent anti-inflammatory responses. Lm infection enhanced differentiation of proinflammatory Th17 cells, which may also exacerbate pathological responses during listeriosis.http://www.sciencedirect.com/science/article/pii/S1438422120300072Listeria monocytogenesAgingIL-17IFN-γCD39/CD73Treg cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Samiul Alam Christopher Cavanaugh Marion Pereira Uma Babu Kristina Williams |
spellingShingle |
M. Samiul Alam Christopher Cavanaugh Marion Pereira Uma Babu Kristina Williams Susceptibility of aging mice to listeriosis: Role of anti-inflammatory responses with enhanced Treg-cell expression of CD39/CD73 and Th-17 cells International Journal of Medical Microbiology Listeria monocytogenes Aging IL-17 IFN-γ CD39/CD73 Treg cells |
author_facet |
M. Samiul Alam Christopher Cavanaugh Marion Pereira Uma Babu Kristina Williams |
author_sort |
M. Samiul Alam |
title |
Susceptibility of aging mice to listeriosis: Role of anti-inflammatory responses with enhanced Treg-cell expression of CD39/CD73 and Th-17 cells |
title_short |
Susceptibility of aging mice to listeriosis: Role of anti-inflammatory responses with enhanced Treg-cell expression of CD39/CD73 and Th-17 cells |
title_full |
Susceptibility of aging mice to listeriosis: Role of anti-inflammatory responses with enhanced Treg-cell expression of CD39/CD73 and Th-17 cells |
title_fullStr |
Susceptibility of aging mice to listeriosis: Role of anti-inflammatory responses with enhanced Treg-cell expression of CD39/CD73 and Th-17 cells |
title_full_unstemmed |
Susceptibility of aging mice to listeriosis: Role of anti-inflammatory responses with enhanced Treg-cell expression of CD39/CD73 and Th-17 cells |
title_sort |
susceptibility of aging mice to listeriosis: role of anti-inflammatory responses with enhanced treg-cell expression of cd39/cd73 and th-17 cells |
publisher |
Elsevier |
series |
International Journal of Medical Microbiology |
issn |
1438-4221 |
publishDate |
2020-02-01 |
description |
Foodborne Listeria monocytogenes (Lm) causes serious illness and death in immunosuppressed hosts, including the elderly population. We investigated Lm susceptibility and inflammatory cytokines in geriatric mice. Young-adult and old mice were gavaged with a Lm strain Lmo-InlAm. Tissues were assayed for Lm burden and splenocytes were analyzed for Th1/Th2/Th17/Treg responses and expression of CD39 and CD73. Old Lm-infected mice lost body-weight dose-dependently, had higher Lm colonization, and showed higher inflammatory responses than Lm-infected young-adult mice. After infection, IL-17 levels increased significantly in old mice whereas IFN-γ levels were unchanged. Levels of IL-10 and Treg cells were increased in infected old mice as compared to infected young-adult mice. Age-dependent enhanced expression of CD39/CD73 was observed in purified Treg prior to infection, suggesting increased baseline adenosine production in old mice. Lm lysate-treated splenocytes from older mice produced significantly higher levels of IL-10, IL17, and IL-1β, produced less IFN-γ and IL-2, and proliferated less than splenocytes from young-adult mice. Data suggests that older mice maybe more susceptible to Lm infection due to an imbalance of Th cell responses with disproportionate and persistent anti-inflammatory responses. Lm infection enhanced differentiation of proinflammatory Th17 cells, which may also exacerbate pathological responses during listeriosis. |
topic |
Listeria monocytogenes Aging IL-17 IFN-γ CD39/CD73 Treg cells |
url |
http://www.sciencedirect.com/science/article/pii/S1438422120300072 |
work_keys_str_mv |
AT msamiulalam susceptibilityofagingmicetolisteriosisroleofantiinflammatoryresponseswithenhancedtregcellexpressionofcd39cd73andth17cells AT christophercavanaugh susceptibilityofagingmicetolisteriosisroleofantiinflammatoryresponseswithenhancedtregcellexpressionofcd39cd73andth17cells AT marionpereira susceptibilityofagingmicetolisteriosisroleofantiinflammatoryresponseswithenhancedtregcellexpressionofcd39cd73andth17cells AT umababu susceptibilityofagingmicetolisteriosisroleofantiinflammatoryresponseswithenhancedtregcellexpressionofcd39cd73andth17cells AT kristinawilliams susceptibilityofagingmicetolisteriosisroleofantiinflammatoryresponseswithenhancedtregcellexpressionofcd39cd73andth17cells |
_version_ |
1721397152851165184 |