Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae

Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae

Abstract Objective The objective was to investigate fixative solutions: 3.7% formaldehyde, 4% paraformaldehyde, 4% paraformaldehyde in the cytoskeletal buffer and 4% paraformaldehyde in PHEM buffer (containing PIPES, HEPES, EGTA and MgCl2), applicable for immunofluorescence assay. Results Herein we...

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Bibliographic Details
Main Authors: Monika Danchenko, Lucia Csaderova, Pierre Edouard Fournier, Zuzana Sekeyova
Format: Article
Language:English
Published: BMC 2019-10-01
Series:BMC Research Notes
Subjects:
Immunofluorescence assay
Rickettsiae
Cytoskeleton fixation
Cell culture
Actin-based motility
Online Access:http://link.springer.com/article/10.1186/s13104-019-4699-9

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http://link.springer.com/article/10.1186/s13104-019-4699-9

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