High-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts

The quantification of bacteria in cell culture infection models is of paramount importance for the characterization of host-pathogen interactions and pathogenicity factors involved. The standard to enumerate bacteria in these assays is plating of a dilution series on solid agar and counting of the r...

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Bibliographic Details
Main Authors: Stefanie Hoffmann, Steffi Walter, Anne-Kathrin Blume, Stephan Fuchs, Christiane Schmidt, Annemarie Scholz, Roman G. Gerlach
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fcimb.2018.00043/full