A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions
Abstract Background Adult bone marrow contains stem cells that replenish the myeloid and lymphoid lineages. A subset of human and mouse CD34+ bone marrow stem cells can be propagated in culture to autonomously express embryonic stem cell genes and embryonic germ layer lineage genes. The current stud...
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doaj-5283cdddce5b4a21a16db46a8269c5f62020-11-25T00:18:33ZengBMCStem Cell Research & Therapy1757-65122018-05-019111010.1186/s13287-018-0858-5A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditionsTapas Makar0Vamshi K. Nimmagadda1Poornachander R. Guda2Brian Hampton3Weiliang Huang4Maureen A. Kane5Paul S. Fishman6Bernard Pessac7Christopher T. Bever8David Trisler9Department of Neurology, University of Maryland School of MedicineDepartment of Neurology, University of Maryland School of MedicineDepartment of Neurology, University of Maryland School of MedicineProtein Analysis Laboratory, Center for Vascular and Inflammatory Diseases, University of Maryland School of MedicineUniversity of Maryland School of Pharmacy, Department of Pharmaceutical SciencesUniversity of Maryland School of Pharmacy, Department of Pharmaceutical SciencesDepartment of Neurology, University of Maryland School of MedicineCNRS UMR 8118, Université Paris DescartesDepartment of Neurology, University of Maryland School of MedicineDepartment of Neurology, University of Maryland School of MedicineAbstract Background Adult bone marrow contains stem cells that replenish the myeloid and lymphoid lineages. A subset of human and mouse CD34+ bone marrow stem cells can be propagated in culture to autonomously express embryonic stem cell genes and embryonic germ layer lineage genes. The current study was undertaken to determine whether these CD34+ stem cells could be obtained from human blood, whether gene expression could be modulated by culture conditions and whether the cells produce insulin. Methods Human peripheral blood buffy coat cells and mobilized CD34+ cells from human blood and from blood from C57Bl/6 J mice were cultured in hybridoma medium or neural stem cell induction medium supplemented with interleukin (IL)-3, IL-6, and stem cell factor (SCF). Changes in mRNA and protein expression were assessed by Western blot analysis and by immunohistochemistry. Mass spectrometry was used to assess insulin production. Results We were able to culture CD34+ cells expressing embryonic stem cell and embryonic germ layer lineage genes from adult human peripheral blood after standard mobilization procedures and from mouse peripheral blood. Gene expression could be modulated by culture conditions, and the cells produced insulin in culture. Conclusion These results suggest a practical method for obtaining large numbers of CD34+ cells from humans to allow studies on their potential to differentiate into other cell types.http://link.springer.com/article/10.1186/s13287-018-0858-5CD34+ stem cellsHuman bone marrow stem cellESC genesLineage genesPancreasInsulin |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tapas Makar Vamshi K. Nimmagadda Poornachander R. Guda Brian Hampton Weiliang Huang Maureen A. Kane Paul S. Fishman Bernard Pessac Christopher T. Bever David Trisler |
spellingShingle |
Tapas Makar Vamshi K. Nimmagadda Poornachander R. Guda Brian Hampton Weiliang Huang Maureen A. Kane Paul S. Fishman Bernard Pessac Christopher T. Bever David Trisler A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions Stem Cell Research & Therapy CD34+ stem cells Human bone marrow stem cell ESC genes Lineage genes Pancreas Insulin |
author_facet |
Tapas Makar Vamshi K. Nimmagadda Poornachander R. Guda Brian Hampton Weiliang Huang Maureen A. Kane Paul S. Fishman Bernard Pessac Christopher T. Bever David Trisler |
author_sort |
Tapas Makar |
title |
A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions |
title_short |
A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions |
title_full |
A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions |
title_fullStr |
A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions |
title_full_unstemmed |
A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions |
title_sort |
subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2018-05-01 |
description |
Abstract Background Adult bone marrow contains stem cells that replenish the myeloid and lymphoid lineages. A subset of human and mouse CD34+ bone marrow stem cells can be propagated in culture to autonomously express embryonic stem cell genes and embryonic germ layer lineage genes. The current study was undertaken to determine whether these CD34+ stem cells could be obtained from human blood, whether gene expression could be modulated by culture conditions and whether the cells produce insulin. Methods Human peripheral blood buffy coat cells and mobilized CD34+ cells from human blood and from blood from C57Bl/6 J mice were cultured in hybridoma medium or neural stem cell induction medium supplemented with interleukin (IL)-3, IL-6, and stem cell factor (SCF). Changes in mRNA and protein expression were assessed by Western blot analysis and by immunohistochemistry. Mass spectrometry was used to assess insulin production. Results We were able to culture CD34+ cells expressing embryonic stem cell and embryonic germ layer lineage genes from adult human peripheral blood after standard mobilization procedures and from mouse peripheral blood. Gene expression could be modulated by culture conditions, and the cells produced insulin in culture. Conclusion These results suggest a practical method for obtaining large numbers of CD34+ cells from humans to allow studies on their potential to differentiate into other cell types. |
topic |
CD34+ stem cells Human bone marrow stem cell ESC genes Lineage genes Pancreas Insulin |
url |
http://link.springer.com/article/10.1186/s13287-018-0858-5 |
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